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The second reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as items (Fig. 9) - circularly polarized luminescence. As drawn in Fig - my explanation https://www.slideshare.net/julieanndesalorenz30. 9, the response may also involve electron transfer from H4B to the Fe, IIO2 intermediate


l9 As revealed in Fig. 9, this could be since the H4B extreme acts as an electron sink in a later point in the reaction, so regarding allow NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.